VEGA Grants

VEGA Grants

VEGA 1/0873/1: Development of novel separation methods alongside with MS/MS detection for optimalization in course of non-specifif treatment of inflammatory bowel diseases by tiopurines

Non-specifif inflammatory bowel diseases (IBD) fall into special class of disease, affecticng prevalently gastrointestinal tract and manifest predominantly in juvenile ages. Tiopurines are very effective and relatively cheap treatment modality of IBD. Their intake is however limited by the occurrence of adverse effects in 10% of patients and the treatment effect depends on the individual patient's metabolic profile

Monitoring of treatment by control of levels of thiopurine pharmacodynamically active metabolite seems the way forward to improve response to treatment, and also minimize the occurrence of side effects. The clinical benefit of treatment monitoring but is now limited analytical methods used. To date no complete by the highly sensitive and specific method for the simultaneous determination of the nucleotide tiopurínových-metabolites, such as the multi-dimensional HPLC-MS / MS. The conrnestone of the project leds in a detailed analysis of the thiopurine metabolitical degradation observed in IBD patients.